What is the color of OG6 solution?
Papanicolaou’s solution 2a Orange G solution (OG6) gives a pale, yellow-orange staining result with mature and keratinized squamous cells.
How do you prepare OG6?
1) Rinse in 70% alcohol, 50% alcohol and distilled water. 2) Stain in Harris haematoxylin (without acetic acid) for 5 -10 minutes. 3) Rinse in distilled water. 4) Rinse 3 or 4 times in 0.5% aqueous solution of hydrochloric acid.
What is the next reagent used after OG6 in Paps staining procedure?
6. Post-OG and post-EA alcohol is replaced by 0.5% of acetic acid. Acid pH keeps acid dyes in cells as 95% alcohol. In addition, dilute acetic acid solution is too weak to remove hematoxylin as HCl used to differentiate in regressive procedure.